In present study preliminary screening was carried on Cyperus routunds crude extract which showed the presence of tannins, sapanins, carbohydrate in colour reaction method while the alkaloids, protein and sterol were absent. Tail flick method was used for the determination of analgesic activity. The temperature and duration were 51 ± 1C° and 0, 1, 2, 3 and 4 hours respectively. The test drug was used in the concentrations of 300 and 500mg/kg orally. Antimicrobial activity were carried out on human pathogens bacteria such as Morexilla catarhalis, Escherichia coli, klebsiella Pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus, acineto bacter and fungi Candida albicans and Aspergillus niger. Excellent, moderate low and no activity were found on these organism such as 133.3% in K. Pneumoniae as compared to standard drug amoxicillin 20µg/ml and 70% ethanol (as fungicide). In case of A. niger and S. aureus 90 and 70 % inhibition was observed respectively while the crude extract showed low inhabitation that is 46.66, 37.5 and 33.3% in E coli, P. aeruginosa and M. catarhalis respectively. No zone of inhibition was observed in Acineto bacter and C. albican. Haemagglutination activity test is suitable for determining the toxic effect on different human blood group ( A+ , B+, AB+, O+, A-, B-, AB- and O-). This test was carried out in different concentration of crude extract that is 0.3125, 0.625, 1.25 and 5mg/ml. Significant result were obtained in all groups at higher concentrations. A quick and low cost toxicity test (Brine shrimp bioassay) was used to find out toxic action of drug in comparison to Etoposide standard drug (LD50 =7.4625). The drug showed non toxic significant effects at 10, 100, 1000 µg/ml concentrations

Keywords : Cyperus rotundus, Antimicrobial, analgesic, cytotoxicity.